Abstract
Arch-cruciform DNA are self-assembled on AuNPs/VS2 scaffold as a highly sensitive and selective electrochemical biosensor for michigan cancer foundation-7 (MCF-7) breast cancer cells. In the construction, arch DNA is formed using two single-strand DNA sequences embedded with the aptamer for MCF-7 cells. In the absence of MCF-7 cells, a cruciform DNA labeled with three terminal biotin is bound to the top of arch DNA, which further combines with streptavidin-labeled horseradish peroxidase (HRP) to catalyze the hydroquinone-H2O2 reaction on the electrode surface. The presence of MCF-7 cells can release the cruciform DNA and reduce the amount of immobilized HRP, thus effectively inhibiting enzyme-mediated electrocatalysis. The electrochemical response of the sensor is negatively correlated with the concentration of MCF-7 cells, with a linear range of 10~1 × 105 cells/mL, and a limit of detection as low as 5 cells/mL (S/N = 3). Through two-dimensional materials and enzyme-based dual signal amplification, this biosensor may pave new ways for the highly sensitive detection of tumor cells in real samples.
Highlights
Breast cancer is the fifth leading cause of death among all cancers, and it is the most common non-skin cancer in women [1,2]
The breast cancer cell line can be classified based on the status of three important receptors, including estrogen receptor (ER), progesterone receptor (PR), and human epithelial receptor-2 (HER2) [3]
michigan cancer foundation-7 (MCF-7) (ER+PR+HER2-), a typical breast cancer cell line, accounts for more than two-thirds of the cell lines used in related studies, along with T47-D and MDA-MB-231 cells, which have been widely used for breast cancer modelling
Summary
Breast cancer is the fifth leading cause of death among all cancers, and it is the most common non-skin cancer in women [1,2]. There are several common breast cancer screening methods, including computed tomography (CT) [7,8], magnetic resonance imaging (MRI) [9,10], positron emission computed tomography (PET) [11,12] and flow cytometry [13]. These techniques are usually expensive and time-consuming, and can lead to false-positive or negative results due to limited resolution [14]. There are numerous methods to detect MCF-7, including electrochemistry [15], electrochemiluminescence [16], colorimetry [17] and photoelectrochemical methods [18]
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