Abstract
In this study, a simple and sensitive aptamer-based colorimetric method for the detection of Ochratoxin A by using gold nanoparticles (AuNPs) has been developed. In this assay, unmodified gold nanoparticles (AuNPs) were used as probes with a 36-mer aptamer as recognition element. In the absence of ochratoxin A, free aptamer could be adsorbed onto the surface of AuNPs and protect AuNPs from aggregation even with high concentrations of salt. The salt-induced aggregation of AuNPs was caused by the specific recognition of aptamers with OTA. Under optimum conditions, calibration modeling showed that the analytical linear range covered from 32 to 1024 ng/mL and the detection limit of 20 ng/mL was realized successfully. This proposed colorimetric bio-assay also showed high selectivity over other antibody based methods. Meanwhile, this strategy was further used to determine the concentrations of ochratoxin A in white wine sample with satisfying recovery rates.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
