Abstract
A comparative study of various approaches to speckle reduction showed that the use of a liquid crystal-based speckle reducer did not allow complete elimination of speckles. The use of mechanical devices for blurring of the speckle pattern in the field of view turned out to be more efficient in the case of quantitative luminescent microscopy. Virtually complete reduction of speckles was observed when a device that combines a ring-shaped fiber-optic light source and a vibration unit that shifts the butt-ends of optical fibers relative to the laser diode during measurement was used. This speckle-reduction approach was successfully used in microarray analysis.
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