Abstract
Currently, the problem of antibiotic resistance of opportunistic and pathogenic microorganisms is extremely urgent. In order to find new effective natural antibiotics, it is necessary to intensify the search process. In the gradual selection of the most promising producers, we introduced the stage of determining the antibiotic activity of the culture fluid of the studied natural strains against the clinical isolates of hospital microorganisms with multiple resistance to medical antibiotics. Determining the species affiliation of potential producers allows to select those producers of a particular species that differ in the antimicrobial spectrum of activity from those described in the literature. Four strains of actinomycetes that showed activity against resistant clinical isolates of yeast Candida albicans, C.famata, C.parapsilosis and Cryptococcus neoformans were selected, namely: Nocardia soli INA 01217, Streptomyces bottropensis INA 01214, S.chromofuscus INA 01211 and S.netropsis INA 01190. The N.soli INA 01217 strain also shows antibiotic activity against the Gram-negative bacterium Escherichia coli ATCC 25922. These strains of actinobacterial producers were selected for subsequent chemical studies of the antimicrobial compounds formed by them.
Highlights
ЭКСПЕРИМЕНТАЛЬНЫЕ СТАТЬИ activity against resistant clinical isolates of yeast Candida albicans, C.famata, C.parapsilosis and Cryptococcus neoformans were selected, namely: Nocardia soli INA 01217, Streptomyces bottropensis INA 01214, S.chromofuscus INA 01211 and S.netropsis INA 01190
In the gradual selection of the most promising producers, we introduced the stage of determining the antibiotic activity of the culture fluid of the studied natural strains against the clinical isolates of hospital microorganisms with multiple resistance to medical antibiotics
The N.soli INA 01217 strain shows antibiotic activity against the Gram-negative bacterium Escherichia coli ATCC 25922. These strains of actinobacterial producers were selected for subsequent chemical studies of the antimicrobial compounds formed by them
Summary
Агаровые среды для роста и идентификации актиномицетов, а также жидкие культуральные среды, разработаны для продуцентов антибиотиков в ФГБНУ «НИИНА» им. Для хранения и поддержания всех микроорганизмов использовали среду No2 Гаузе. Актиномицеты инкубировали при 28°C в течение 10–12 сут. Грибные тест-штаммы Aspergillus niger ИНА 00760 и Saccharomyces cerevisiae ИНА 01129 инкубировали при 28°C в течение 48 и 24 ч, соответственно. На первой стадии использовали жидкую среду No 2 Гаузе, которую засевали кусочком агаровой среды размером примерно 1 см с поверхностным ростом актиномицета. После 4 сут культивирования для второй стадии полученную культуральную жидкость в количестве 5 мл вносили в колбы со свежей средой. Отбор проб для определения антибиотической активности проводили на четвёртый и седьмой день роста. Коллекционные штаммы грамположительных и грамотрицательных бактерий, грибов, а также клинические изоляты микроорганизмов с различной устойчивостью к антибиотикам, использовали для определения антимикробной активности. Streptomyces antibioticus ИНА 01148 S.netropsis ИНА 01190 S.chromofuscus ИНА 01211 S.hydrogenans ИНА 01212 S.bottropensis ИНА 01214 S.hydrogenans ИНА 01215 Nocardia soli ИНА 01217 S.lusitanus ИНА 01218 S.peucetius ИНА 01255 S.chartreusis ИНА 01259
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