Abstract
The aim of this study was to determine the impending antioxidant properties of different extracts of crude methanolic extract (CME) of leaves of Lannea coromandelica (L. coromandelica) and its two ethyl acetate (EAF) and aqueous (AqF) subfractions by employing various established in vitro systems and estimation of total phenolic and flavonoid content. The results showed that extract and fractions possessed strong antioxidant activity in vitro and among them, EAF had the strongest antioxidant activity. EAF was confirmed for its highest phenolic content, total flavonoid contents, and total antioxidant capacity. The EAF was found to show remarkable scavenging activity on 2,2-diphenylpicrylhydrazyl (DPPH) (EC50 63.9 ± 0.64 µg/mL), superoxide radical (EC50 8.2 ± 0.12 mg/mL), and Fe2+ chelating activity (EC50 6.2 ± 0.09 mg/mL). Based on our in vitro results, EAF was investigated for in vivo antioxidant assay. Intragastric administration of the EAF can significantly increase levels of superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), and glutathione peroxidase (GSH-Px) levels, and decrease malondialdehyde (MDA) content in the liver and kidney of CCl4-intoxicated rats. These new evidences show that L. coromandelica bared antioxidant activity.
Highlights
In recent times peoples are paying their attention towards the importance of dietary antioxidant components for the betterment of human health due to the apprehension about the side effects of synthetic antioxidants
Consumption of a variety of phenolic compounds may lower the risk of serious health disorders due to considerable resistance to the oxidative damage caused by the reactive oxygen species (ROS) [2,3,4]
Preliminary phytochemical screening of extract and fractions of L. coromandelica showed the presence of alkaloids, steroid, triterpenes, flavonoid, and polyphenolics compounds (Table 1)
Summary
In recent times peoples are paying their attention towards the importance of dietary antioxidant components for the betterment of human health due to the apprehension about the side effects of synthetic antioxidants. Herbals, and medicinal plants are playing an important role as powerful antioxidants due to the presence abundance of polyphenolic compounds [1]. Plant phenolic compounds are the products of secondary metabolism, ubiquitously present in the plant kingdom with interesting properties for animal or human health. Consumption of a variety of phenolic compounds may lower the risk of serious health disorders (chronical and degenerative diseases) due to considerable resistance to the oxidative damage caused by the ROS [2,3,4]. Overexcited antioxidant enzyme system (superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx)/oxidized glutathione reductase (GSSGRD)
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