Abstract
Pleurotus florida has been widely used for nutritional and medicinal purposes. The present study was conducted to evaluate the antioxidant and anti-inflammatory effects of the fruiting bodies of P. florida extracted with acetone, methanol, and hot water. The antioxidant activities of the acetone and methanol extracts of P. florida showed stronger inhibition of β-carotene-linoleic acid compared to that of the hot water extract. The acetone extract (8 mg/mL) showed a high reducing power of 1.86. The acetone and methanol extracts showed more effective DPPH radical scavenging activities than the hot water extract. The chelating effect of the extracts at lower concentrations was significantly effective compared to that of the positive control. Thirteen phenolic compounds were detected from acetonitrile and hydrochloric acid solvent extracts. Nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) expression in lipolysaccahride (LPS) stimulated RAW 264.7 cells, a murine macrophage cell line, were inhibited significantly by the mushroom extracts in a concentration dependent manner. The anti-inflammatory activity on carrageenan-induced edema in the rat hind-paw reduced significantly by the mushroom extracts. Therefore, we have demonstrated that P. florida fruiting bodies possess antioxidant and anti-inflammatory activites related to their inhibitory activities on NO production, iNOS protein expression, and carrageenan-induced paw edema in rats. The results suggest that the fruiting bodies of P. florida are a good source of natural antioxidant and anti-inflammatory agents.
Highlights
Pleurotus florida is an edible mushroom that belongs to the family Pleurotaceae, order Agaricales, Basidiomycota
The antioxidant activity of carotenoids is based on the formation of adducts of the carotenoids with the free radicals from linoleic acid
It is probable that the antioxidative components in the mushroom extracts can reduce the extent of β-carotene destruction by neutralizing the linoleate free radical and other free radicals formed in the system [9]
Summary
Pleurotus florida is an edible mushroom that belongs to the family Pleurotaceae, order Agaricales, Basidiomycota This mushroom produces various biologically active molecules and novel enzymes [1]. Inflammation is a physiological immune response of the host against internal and external stimuli such as infection, tissue injury, or noxious stimulants; the over-production of inflammatory products can lead to a series of vascular and cellular reactions [4]. Inflammatory markers such as ROS, reactive nitrogen species, tumor necrosis factor-α, interleukin (IL)-1, IL-6, and cyclooxygenase (COX). The phenolic compound profiles of the mushroom extracts were determined
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