Applying Spermidine for Differential Responses of Antioxidant Enzymes in Cucumber Subjected to Short-term Salinity

Abstract

To examine whether spermidine (SPD) modifies plant antioxidant enzyme expression in response to short-term salt stress, cucumber (Cucumis sativus) seedlings were treated with NaCl in the presence or absence of SPD for 3 days. Compared with untreated control plants, free radical production and malondialdehyde content in leaves and roots increased significantly and plant growth was suppressed under 50 mm NaCl stress. Exogenous SPD sprayed on leaves at a concentration of 1 mm alleviated salinity-mediated growth reduction. Salt stress caused a consistent increase in soluble protein content, as well as peroxidase (POD) and superoxide dismutase (SOD) activities in cucumber seedlings. By native polyacrylamide gel electrophoresis, five POD isozymes were detected in cucumber seedling leaves, and seven in roots. We detected five SOD isozymes in leaves and four in roots, and two catalase (CAT) isozymes in leaves and two in roots. Our results indicate that salt stress induced the expression of POD and SOD isozymes in cucumber seedlings, but inhibited the expression of CAT isozymes in roots. Application of exogenous SPD further increased POD and SOD expression and activity, and led to the differential regulation of CAT in leaves and roots. These data show that antioxidant enzymes, especially POD and SOD, appear to protect cucumber seedlings against stress-related damage, and they appear to function as the molecular mechanisms underlying the response of cucumber seedlings to salinity. Moreover, SPD has potential to scavenge directly free radical and to alleviate growth inhibition and promote the activity and expression of antioxidant system enzymes in cucumber seedlings under short-term salt stress.