Abstract
The Genome Resource Bank (GRB) is a repository of frozen biological material, including semen and embryos. Cryobanking is used in combination with modern reproductive technologies such as rederivation, in vitro culture and embryo transfer. Thirteen mouse and rat strains have been re-derived and 32 are kept frozen in the cryostorage at the Institute of Cytology and Genetics, Novosibirsk. Some other laboratory animal species have been cryopreserved as well. Embryos of two hamster species (Djungarian and Campbell’s) in the genus Phodopus were cryopreserved and the viability of thawed embryos was proved by their successful development in vitro and in vivo (by transfer to a recipient). A positive effect of the granulocyte-macrophage colony-stimulating factor (GM-CSF) was demonstrated with both these Phodopus species. Furthermore, semen of Djungarian (Phodopus sungorus) and Campbell’s (Phodopus campbelli) hamsters, domestic cat (Felis catus), amur cat (Prionailurus bengalensis euptilurus) and bobcat (Lynx rufus) was frozen and cryopreserved. Double staining by SYBR Green/PI and subsequent confocal microscopy demonstrated that more than 40 % of amur cat semen retained viability after cryopreservation. This is the world’s first reported successful freezing of semen of this wild felid (Prionailurus bengalensis euptilurus). This article reviews the results and discusses prospects of using reproductive technologies for conservation of laboratory species.
Highlights
The Genome Resource Bank (GRB) is a repository of frozen biological material, including semen and embryos
Cryobanking is used in combination with modern reproductive technologies such as rederivation, in vitro culture and embryo transfer
Embryos of two hamster species (Djungarian and Campbell’s) in the genus Phodopus were cryopreserved and the viability of thawed embryos was proved by their successful development in vitro and in vivo
Summary
Амстиславский С.Я., Брусенцев Е.Ю., Абрамова Т.О., Рагаева Д.С., Рожкова И.Н., Игонина Т.Н., Кизилова Е.А., Напримеров В.А., Феоктистова Н.Ю. Amstislavsky S.Ya., Brusentsev E.Yu., Abramova T.O., Ragaeva D.S., Rozhko va I.N., Igonina T.N., Kizilova E.A., Naprimerov V.A., Feoktistoiva N.Yu. Applying reproductive technologies and genome resource banking to laboratory animals. По отношению ко многим видам млекопитающих, в том числе и некоторым видам лабораторных грызунов, способы замораживания эмбрионов и/или семени пока находятся лишь в стадии разработки и требуют тщательного изучения биологии конкретного вида (Амстиславский и др., 2015). Конкретными задачами являются: 1) общий обзор работы, проведенной в ИЦиГ СО РАН по созданию криобанка эмбрионов мышей и крыс; 2) анализ текущего состояния исследований по созданию криобанков эмбрионов и семени других лабораторных животных, помимо мышей и крыс, на примере проведенного нами цикла экспериментов с хомячками джунгарским и Кэмпбелла; 3) анализ текущего состояния исследований по сохранению видов кошачьих путем замораживания семени. Сублиний и стоков мышей и крыс, переведенных в SPF-статус посредством репродуктивных технологий или архивированных в криобанке в ИЦиГ СО РАН
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