Abstract

This study is a first attempt to find different types of RNA in lactating buffalo's milk somatic cells. The molecular factors that regulate lactation need to be identified and understood in order to help milk production. By using data mining techniques, patterns and information hidden within a dataset can be identified. In order to detect the RNA, data of 12 samples of buffalo milk somatic cells were analyzed. For extraction of diverse RNAs COMPSRA (COMprehensive Platform for Small RNA Analysis) pipeline was used. We were able to identify several miRNAs, piRNAs, snRNAs, snoRNAs, circRNAs and tRNAs in buffalo milk somatic cells. circRNAs ranked highest among all the samples in our dataset, followed by piRNAs and then miRNAs. Understanding the RNA regulators of lactation will improve and facilitate management of buffalo milk production. Furthermore, our study contributes towards a complete annotation of the buffalo genome.

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