Applications of the CRISPR-Cas9 system in the treatment of KRAS mutated lung cancer

Abstract

Lung cancer is currently the most prevailed cancer in men, and the second most prevailed cancer in women. It is by far the most fatal cancer with around 21% of overall death from cancer. It is very important to seek a way to treat lung cancers, since too many people died for lung cancers, too many families broke up and collapsed, and too many people are left with broken spirituality and heart. On the other hand, the successful treatment of lung cancer using method of gene editing could symbolize for the success of the appliance of gene editing on other deadly cancers. The main type of gene editing method that will be utilized is CRISPR-Cas9. This is a relatively new type of gene editing technology, thus it meant that this is the most applicable when coming to cost and precision, whereas it could also mean that it is still in its preform and hasnt been widely performed and tested. This review offers a brief introduction on lung cancer, summarizes CRISPR-Cas9s mechanism and provides the comparison of CRISPR-Cas9 on other gene editing technologies such as ZFNs and TALENs, sets up a plan to detect KRAS mutated lung cancer and apply CRISPR-Cas9 to treat KRAS mutated lung cancer in human cell lines and in chimpanzees.