Applications of Single-Cell RNA Sequencing in Cardiovascular Research.

Yu Fan,Ke Xu,Lei Ye,Xiaodong Fu,Jingyan Li,Xuexue Liu,Guang Li,Han Zhou

doi:10.3389/fcell.2021.810232

Abstract

In recent years, cardiovascular disease (CVD) continues to be the leading cause of global disease burden. Extensive efforts have been made across basic, translational, and clinical research domains to curb the CVD epidemic and improve the health of the population. The successful completion of the Human Genome Project catapulted sequencing technology into the mainstream and aroused the interests of clinicians and scientific researchers alike. Advances in single-cell RNA sequencing (scRNA-seq), which is based on the transcriptional phenotypes of individual cells, have enabled the investigation of cellular fate, heterogeneity, and cell–cell interactions, as well as cell lineage determination, at a single-cell resolution. In this review, we summarize recent findings on the embryological development of the cardiovascular system and the pathogenesis and treatment of cardiovascular disease, as revealed by scRNA-seq technology. In particular, we discuss how scRNA-seq can help identify potential targets for the treatment of cardiovascular diseases and conclude with future perspectives for scRNA-seq.

Highlights

Cardiovascular disease (CVD), primarily ischemic heart disease and stroke, remains the leading cause of mortality and morbidity across the world (GBD 2019 Diseases and Injuries Collaborators, 2020)
Recent evidence indicates that the prevalence of total CVD has continued to increase, nearly doubling from 271 million in 1990 to 523 million in 2019
The promotion of cardiovascular health remains an important task for clinicians and scientific researchers

Summary

INTRODUCTION

Cardiovascular disease (CVD), primarily ischemic heart disease and stroke, remains the leading cause of mortality and morbidity across the world (GBD 2019 Diseases and Injuries Collaborators, 2020). The burden of CVD continues its decade-long rise in almost all countries except high-income countries. In most low- and middle-income countries, the overall burden of CVD, in terms of morbidity and mortality, is higher than that in high-income countries (Roth et al, 2020). Single-cell sequencing investigates biodiversity and heterogeneity at a single-cell resolution (Grün and van Oudenaarden, 2015). Since it was rated as “The Method of the Year” by Nature Methods in 2013, multiple single-cell sequencing methods and platforms have been developed for various

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Novel cardiac progenitor marker

Early heart failure

Cell phenotypic transformation

AUTHOR CONTRIBUTIONS