Abstract
Pullulan, a microbial polysaccharide, was employed with polyethylene glycol (PEG) to form an aqueous two-phase system. The phase diagram of the PEG-Pullulan systems as well as the partition of enzymes depended strongly on the molecular weight of PEG. This behavior provided a basis for the separation of cellulase from β-galactosidase. The extractive hydrolysis of lactose, using a commercial preparation of β-galactosidase, could also be performed in a PEG-Pullulan system (16% PEG 1400, 14% Pullulan). The enzyme was effectively contained in the bottom phase while a high percentage of the glucose produced could be removed in the top phase. The enzyme activity was preserved very well after several repeated hydrolyses.
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