Applications of near-infrared spectroscopy in "anaerobic" diagnostics - SmO2 kinetics reflect PCr dephosphorylation and correlate with maximal lactate accumulation and maximal pedalling rate.

Abstract

We investigated the relationship of the time-dependent behaviour of muscle oxygen saturation SmO2(t), phosphagen energy supply WPCr(t) and blood lactate accumulation ΔBLC(t) during a 60-s all-out cycling sprint and tested SmO2(t) for correlations with the end of the fatigue-free state tFf, maximal pedalling rate PRmax and maximal blood lactate accumulation rate v̇Lamax. Nine male elite track cyclists performed four maximal sprints (3, 8, 12, 60 s) on a cycle ergometer. Crank force and cadence were monitored continuously to determine PRmax and tFf based on force-velocity profiles. SmO2 of the vastus lateralis muscle and respiratory gases were measured until the 30th minute after exercise. WPCr was calculated based on the fast component of the post-exercise oxygen uptake for each sprint. Before and for 30 minutes after each sprint, capillary blood samples were taken to determine the associated ΔBLC. Temporal changes of SmO2, WPCr and ΔBLC were analysed via non-linear regression analysis. v̇Lamax was calculated based on ΔBLC(t) as the highest blood lactate accumulation rate. All models showed excellent quality (R2 > 0.95). The time constant of SmO2(t) τSmO2 = 2.93 ± 0.65 s was correlated with the time constant of WPCr(t) τPCr = 3.23 ± 0.67 s (r = 0.790, p < 0.012), v̇Lamax = 0.95 ± 0.18 mmol · l-1 · s-1 (r = 0.768, p < 0.017) and PRmax = 299.51 ± 14.70 rpm (r = -0.670, p < 0.049). tFf was correlated with τSmO2 (r = 0.885, p < 0.001). Our results show a time-dependent reflection of SmO2 kinetics and phosphagen energy contribution during a 60-s maximal cycling sprint. A high v̇Lamax results in a reduction, a high PRmax in an increase of the desaturation rate. The half-life of SmO2 desaturation indicates the end of the fatigue-free state.