Applications of Mechanochemistry for the Synthesis of DNA on Ionic Liquid Supports

Abstract

AbstractThe standard methodology for the synthesis of oligonucleotides is the solid‐phase method. Here, we report the first case of mechanochemistry being directly applied to synthesising a dinucleotide on a soluble support. While mechanochemistry eliminates the need to solubilise a supported oligomer in the solution phase for phosphoramidite coupling, the support still confers the benefit of being able to purify the product by precipitation or by simple liquid‐liquid extractions as needed. Furthermore, this study also explores the use of mechanochemistry in the initial functionalisation of the ionic liquid support with a nucleoside monomer.