Abstract
ABSTRACT Pityrocarpa moniliformis (Benth.) Luckow & R. W. Jobson (Fabaceae) is a native brazilian species with high potential for economic development programs in semiarid regions, mainly related to the production of honey, animal food and firewood. Thus, the objective of this work was to select Inter-Simple Sequence Repeat (ISSR) molecular markers for genetic diversity studies, as well as to test the efficiency of this approach in quantifying the genetic diversity of a natural P. moniliformis population. For this, 28 ISSR molecular markers were tested, evaluating the total number of loci, polymorphism rate and the Polymorphism Information Content (PIC) for the selected primers, the “Marker Index”, and the “Resolving Power”. Genetic diversity parameters (Nei genetic distance and Shannon index) were evaluated for 30 individuals located in Macaíba, Rio Grande do Norte State, Brazil. Seven primers were selected, which provided 74 loci, with 82% being polymorphic, while the PIC value was 0.344. The Nei genetic distance was 0.244, and the Shannon index was 0.374. Therefore, ISSR molecular markers (UBC 827, 840, 844, 857, 859, 860 and 873) are considered efficient in studying the genetic diversity of populations for the selection of matrices and germplasm banks, and may contribute to the conservation and genetic improvement of P. moniliformis populations.
Highlights
IntroductionW. Jobson (Fabaceae) is a tree species that predominantly occurs in dry forests in Northeast Brazil and is popularly known as catanduva
We focus on the ISSR (Inter-Simple Sequence Repeat), which are dominant markers used in genetic diversity studies, with low development cost, a high polymorphism rate and high reproducibility (CHEN et al, 2017)
The objective of this study was to select ISSR molecular markers to be used in genetic diversity studies, as well as to test the efficiency of this approach in quantifying the genetic diversity of a natural P. moniliformis population
Summary
W. Jobson (Fabaceae) is a tree species that predominantly occurs in dry forests in Northeast Brazil and is popularly known as catanduva. Jobson (Fabaceae) is a tree species that predominantly occurs in dry forests in Northeast Brazil and is popularly known as catanduva These native species have social, ecological and economic potential, mainly due to their role in supplying wood for firewood, forage for animal feed, honey production and the recovery of degraded areas (AZERÊDO; PAULA; VALERI, 2011). The wood from P. moniliformis presents excellent mechanical properties, as it demonstrates high density in relation to other currently commercialized species (NASCIMENTO et al, 2015). It has recently been found that its leaves, bark and fruits present high level of antioxidants, which can be used as phytotherapy in fighting against cancer cells (ALVES et al, 2014), and its seeds have secondary metabolites with larvicidal properties against Aedes aegypti L. vectors, which transmit viral diseases (FARIAS et al, 2010)
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