Applications of Fluorescence Correlation Spectroscopy— Particle Size Effect

Abstract

Both computer simulations and experiments have been employed to study the fluorescence correlation spectroscopy autocorrelation function of particles with size comparable to the size of the light beam. When such particles are labeled by several fluorophores the characteristic time of diffusion and the particle number in the light beam are larger than those expected from the existing theory developed for the case of a point size diffuser. The resulting effect is described by simple equations.