Abstract
As an important component that constitutes all the cells and tissues of the human body, protein is involved in most of the biological processes. Inspired by natural protein systems, considerable efforts covering many discipline fields were made to design artificial protein assemblies and put them into application in recent decades. The rapid development of structural DNA nanotechnology offers significant means for protein assemblies and promotes their application. Owing to the programmability, addressability and accurate recognition ability of DNA, many protein assemblies with unprecedented structures and improved functions have been successfully fabricated, consequently creating many brand-new researching fields. In this review, we briefly introduced the DNA-based protein assemblies, and highlighted the limitations in application process and corresponding strategies in four aspects, including biological catalysis, protein detection, biomedicine treatment and other applications.
Highlights
As an important component of organisms, protein is closely concerned with the life activities of almost all the creatures on the earth
Aptamer is an oligonucleotide sequence that can be bound to the target molecule with high affinity and specificity, and it is repeatedly screened from the random oligonucleotide sequence library synthesized in vitro by using systematic evolution of ligands through the exponential enrichment (SELEX) technology
They cover the graphene oxide on the glassy carbon electrode (GCE) via chemical methods, the NH2-funtionalized aptamer was bound to the carboxylated GO through the combination of carboxyl and amino groups
Summary
As an important component of organisms, protein is closely concerned with the life activities of almost all the creatures on the earth. Proteins in nature have extremely complex folding structures They can be combined with some biomacromolecules, such as DNA, RNA and lipids, to form intricate structures [1,2,3,4,5,6], which have shown great application prospects in biocatalysis, chemical detection, biomedicine, food research, etc. DNA strands are often used as programmable motifs with controllable flexibility/rigidity and length, proper candidates for information storage [14,15,16,17] and non-toxic carriers for organisms [18] Among these studies, DNA is especially valued and employed for its characteristics in two respects, including the function of DNA itself, such as aptamers with high affinity and specific binding properties, and the designability of DNA nanostructures. We will briefly introduce the research progress and applications of DNAguided protein assembly in four respects, involving biological catalysis, protein detection, biomedicine treatment and other applications, and estimate the opportunities and challenges in each branch
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
