Abstract
A comparison of the migration behavior of two antibiotics is made between several types of etched columns used in the open tubular format of capillary electrochromatography. The types of columns evaluated are: (1) etched and unmodified; (2) etched and bonded with an octadecyl moiety; (3) etched and bonded with a cholesterol moiety; (4) etched and coated with an anionic surfactant; and (5) etched and coated with a neutral (zwitterionic) surfactant. In addition to evaluating the influence of the stationary phase, the effect of buffer pH is also measured. Each of the antibiotic samples consist of several components and the resolution capabilities of the various columns are tested in order to determine how OTCEC can be optimized for the analysis of small basic compounds.
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