Applications of cellulase enzyme in textile industry purified from Bacillus paramycoides S 5

Abstract

Cellulases are enzymes which breaks down β -1-4-glycosidic bonds present in a complex polysaccharide cellulose. In this study cellulase producing bacteria were isolated from soil samples and efficient cellulase producing bacteria was identified as Bacillus paramycoides S 5 strain by using 16 S rRNA sequencing method. By using the same strain, cellulase enzyme was purified with different methods such as ammonium sulfate precipitation, dialysis, chromatography. The purified enzyme was tested for its applications in textile industry. The applications of cellulase enzymes in txtile industry includes wet processing, biostoning, biopolishing, softening and removal of stain from the clothes. In the present study of applications of purified cellulase enzyme in textile industry, after testing for wet processing and softening of fabrics giving the good biofinishing effects on selected fabric. In another application that is in removal of stain from cloth shows positive effect and found that cellulase has capability to remove stain colour from the cloth, later on cellulase applicability also tested on fabrics with respected to biopolishing which helps to remove the fabrics and microfibrils from the cloth giving a soft appearance to cloth. Finally cellulase was also tested for biostoning.