Abstract
Capillary electrophoresis with capacitively coupled contactless conductivity detector (CE-C4D) is renowned for its numerous advantages, such as compact equipment, low cost of setup and operation, simplicity in functionality, and minimal use of samples and chemical solvents. This method has been applied across various fields, including environmental analysis, forensic science, biochemistry, pharmaceuticals, and food science. In this study, the CE-C4D method was applied to determine the presence of glucosamine, vitamin B6, and certain minerals in dietary supplements. Notably, glucosamine was simultaneously determined with calcium, and vitamin B6 with magnesium. The method was validated to meet the requirements of AOAC. The detection limits achieved for calcium, glucosamine, vitamin B6 and magnesium were 0.05 ppm; 0.50 ppm; 0.10 ppm and 1.00 ppm, respectively. The concurrent determination of active substances with different properties within a single product is crucial, optimizing analytical efficiency and yielding high accuracy for the analysis.
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