Abstract
The ability ammonium trifluoroacetate as an additive for elution of acids and bases from derivatized polysaccharide chiral stationary phases was first observed in the process of developing normal-phase chiral HPLC methods in our lead-generation programs. To demonstrate this ability on a broader scale, chiral HPLC methods containing this additive in the mobile phases were developed to resolve selected acidic, basic, and neutral racemates, which are considered standards in the pharmaceutical industry and for which published methods exist. The mobile phases of these published methods contain acidic and/or basic additives (e.g., trifluoroacetic acid or diethylamine). This article demonstrates the versatility of ammonium trifluoroacetate additive in resolving the enantiomers of acidic and basic racemates on the same derivatized polysaccharide chiral columns. This resolution is achieved without changing the mobile phase between the analysis of acidic and basic racemates and also without observation of stationary-phase "memory effect." This chiral method development strategy can result in significant savings of cost and time.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
