Applications of a Rapid and Sensitive Dengue DUO Rapid Immunochromatographic Test Kit as a Diagnostic Strategy during a Dengue Type 2 Epidemic in an Urban City.

Hsin-I Shih,Chih-Chia Hsieh,Yi-Fang Tu,Chi-Jung Wu,Chih-Hsien Chi,Chih-Hao Lin,Hsiang-Chin Hsu,Tzu-Ching Sung,Chia-Ming Chang,Shamala Devi Sekaran

doi:10.1371/journal.pone.0158437

Abstract

Dengue infection is a major health problem in tropical and subtropical countries. A prospective observational study in a university-affiliated hospital was conducted between August 2015 and September 2015. Patients who visited the emergency department (ED) with a presentation of any symptoms of dengue were eligible for the dengue non-structural protein 1 (NS1), IgM/IgG rapid immunochromatographic tests and real-time polymerase chain reaction (RT-PCR) to evaluate the performance of the rapid tests. Considering the RT-PCR as the gold standard for the dengue diagnosis, the ideal primary results of sensitivity (80–100%), specificity (60–84%), positive predicted value(75%-95%), and negative predicted value (70–100%) suggested that the NS1-based test with or without a combination of IgM and IgG tests have good diagnostic performances in detecting dengue infections, even in the afebrile or elderly populations.

Highlights

Dengue infection caused by four main types of dengue virus (DENV) is a major health problem in tropical and subtropical countries
Clinical presentations of dengue diseases range from asymptomatic or self-limiting dengue fever (DF) to severe dengue characterized by plasma leakage (DHF, grades 1 and 2) that can lead to a life-threatening syndrome (DSS, grades 3 and 4) and severe bleeding and/or severe organ impairment [1]
The International Classification of Diseases, Ninth Revision, Clinical Modification (ICD-9-CM) ICD-9 codes used for the chronic diseases were as follows: The International Classification of Diseases, Ninth Revision, Clinical Modification (ICD-9-CM) ICD-9 codes used for the chronic diseases were as follows: diabetes mellitus (DM)-250, chronic obstructive pulmonary disease (COPD), malignant neoplasms-140-239, chronic kidney disease (CKD)-585, 5839, and chronic liver disease and cirrhosis-571

Summary

Introduction

Dengue infection caused by four main types of dengue virus (DENV) is a major health problem in tropical and subtropical countries. More than 250,000 cases of dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS) are reported from an estimated 50 million dengue infections [1,2,3]. Dengue is transmitted between people by the mosquitoes Aedes. Using a NS1, IgM/IgG Kit during a Dengue Epidemic in a City aegypti and Aedes albopictus, which are found throughout the world. Clinical presentations of dengue diseases range from asymptomatic or self-limiting dengue fever (DF) to severe dengue characterized by plasma leakage (DHF, grades 1 and 2) that can lead to a life-threatening syndrome (DSS, grades 3 and 4) and severe bleeding and/or severe organ impairment [1]. Severe dengue-related fatal cases usually present with DSS, and the mortality of DSS is reportedly 50 times higher than that of dengue patients without DSS [4]

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