Abstract
An Au/Cu bimetallic electrode is investigated as a sensor in amperometric flow-through detection in alkaline solutions. Experimental conditions such as detection mode, pH, copper loading and flow-rate on sensitivity and stability are evaluated. Constant-potential detection at 0.550 V or 0.350 V and pulsed detection modes are tested after anion-exchange chromatography of alditols, carbohydrates and amino acids. The detection limits (S/N=3) of carbohydrates at 0.550 V and 0.350 V ranged between 4.6–9.0 pmol and 1.0–4.0 pmol, respectively. Those of amino acids were 9.0–39 pmol. In pulsed amperometric detection mode, molar sensitivities and detection limits were higher than those obtained in DC mode. Linear dynamic ranges spanned over four (alditols and carbohydrates) and three (amino acids) orders of magnitude. The Au/Cu CME retained 94–96% of its response after several hours operations in flowing streams. As an application, some alditols, carbohydrates and amino acids were determined in soluble coffee and red wine.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.