Abstract
3D cell culture approaches are cell culture methods that provide good visualization of interactions between cells while preserving the natural growth pattern. In recent years, several studies have managed to implement magnetic levitation technology on 3D cell culture applications by either combining cells with magnetic nanoparticles (positive magnetophoresis) or applying a magnetic field directly to the cells in a high-intensity medium (negative magnetophoresis). The positive magnetophoresis technique consists of integrating magnetic nanoparticles into the cells, while the negative magnetophoresis technique consists of levitating the cells without labelling them with magnetic nanoparticles. Magnetic levitation methods can be used to manipulate 3D culture, provide more complex habitats and custom control, or display density data as a sensor.The present review aims to show the advantages, limitations, and promises of magnetic 3D cell culture, along with its application methods, tools, and capabilities as a density sensor. In this context, the promising magnetic levitation technique on 3D cell cultures could be fully utilized in further studies with precise control.
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