Application value of lymphatic filariasis IgG4 antibody specific detection by ELISA

Abstract

Objective To investigate the sensitivity and specificity of ELISA in detecting lymphatic filariasis patients and evaluate the application value. Methods Using double-blind assay, microfilaria positive patients′ serum, serum of patients with other worms, advanced filariasis patients′ filter paper blood, primary hyperlipidemia microfilaria and the elimination of filariasis patients filter paper blood were detected by filarial specific IgG4 ELISA test kits. Results Eighty-six serum of microfilaria positive patients in total.The results of filarial specific IgG4 ELISA was eighty-one cases, the coincidence rate was 94.19%(81/86). Four patients′ of serum and filter were specific IgG4 positive, the antibody level of serum-positive patients was higher than that of paper blood positive patients′.Seropositive patients′ OD values were higher than that of filter paper blood positive patients′, there was no significant difference (Z=-1.826, P>0.05). Serum of ninety-seven patients with other worms, filter paper blood of fourty-eight advanced filariasis patients and filter paper blood of seventy-six microfilaria positive patients who had turnd negative were negative.Furthermore, filter paper bood of three hundred and twelve children who were born in filariasis elimination areas after 1983, and eight hundred cases of three high endemic area were detected negative.Filter paper blood and serum samples in Zaozhuang, Jining, Tai′an where former high degree detections of endemic filariasis elimination were 2744 copies, 2243 copies and 800 copies separately, all samples were negative. Conclusions The filarial specific IgG4 kit is high sensitivity and specificity.The ELISA is suitable for diagnosis and evaluation of filariasis in endemic region, and the filarial specific IgG4 ELISA test kit is simple, fast, and easy to judge the results. Key words: Filariasis; IgG; Enzyme linked immunosorbent assay; Diagnosis