Application Research of Lipid Microsphere Biological Carrier Material on Prescription of Tanshinone II a Lipid Microsphere by Central Composite Design-Response Surface Method

Abstract

To optimize the prescription of tanshinone IIA lipid microspheres by central composite design method, and evaluate the quality. Through evaluating average particle size, stability constant and the encapsulation efficiency of the lipid microsphere to investigate the concentration of the egg yolk lecithin and the poloxamer and the percentage of Soybean oil in the total oil phase which may impact the properties of tanshinone IIA lipid microsphere. Building the mathematic relation between the index and factor by the binomial expression, according to the best mathematical model of the evaluation indicators to describe response surface and then to use response surface method to predict the best prescription which will be analysed. According to the prescription optimization to prepare tanshinone IIA lipid microsphere and evaluate its quality. The results showed that each index of the binomial equation fitting is good, the correlation coefficient of the average particle size, stability constant and the encapsulation efficiency are 0.97, 0.92 and 0.84 respectively. The optimized prescription is as follows: egg yolk lecithin1.38 g, F68 0.19 g, LCT 2 g, MCT 18 g, all the indexes of the optimized prescription are similar to the target values.