Application of zirconium-modified silica gel as a stationary phase in the ion-exclusion chromatography of carboxylic acids: II. Separation of aliphatic carboxylic acids with pyromellitic acid as eluent and with suppressed conductimetric detection

Abstract

The application of zirconium-modified silica gels (Zr-Silica) as stationary phases for ion-exclusion chromatography with conductimetric detection (IEC–CD) for C 1–C 8 aliphatic carboxylic acids (formic, acetic, propionic, butyric, valeric, caproic, heptanoic and caprylic acids) was carried out using pyromellitic acid as the eluent. Zr-Silicas were prepared by the reaction of the silanol group on the surface of silica gel with zirconium tetrabutoxide [Zr(OCH 2CH 2CH 2CH 3) 4] in ethanol solution. An ASRS-Ultra anion self-regenerating suppressor in the K + form was used for the enhancement of conductimetric detector response of these aliphatic carboxylic acids. A Zr-Silica adsorbed on 10 mg zirconium g −1 silica gel was the most suitable stationary phase in IEC–CD for the separation of these aliphatic carboxylic acids. Excellently simultaneous separation and highly sensitive detection for these aliphatic carboxylic acids were achieved in 25 min by IEC–CD with the Zr-Silica column (250×4.6 mm I.D.) and a 0.2 m M pyromellitic acid containing 0.15% heptanol as the eluent.