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Abstract
Tomato yellow leaf curl virus (TYLCV) has significantly impacted the tomato industry around the world, and the use of insecticides and insect nets have not effectively controlled the spread of this pathogen. The tomato line AVTO1227 is highly resistant to TYLCV. In this study, F2 and BC1 populations derived from AVTO1227 and the susceptible line Money maker were used to assess the genetic mechanism underlying TYLCV resistance. We have identified a recessive TYLCV resistance gene, hereby designated as ty-5, which is linked to SlNACI. Genomic DNA pools from resistant and susceptible groups were constructed, and their genomes were resequenced. The ty-5 gene was identified on an interval encompassing the genomic positions 2.22 Mb to 3.19 Mb on tomato chromosome 4. Genotyping using linkage markers further mapped ty-5 within the interval between markers ty5–25 and ty5–29, where only the pelota gene is located. Consequently, pelota was considered as the candidate gene corresponding to ty-5. Two nucleotide transversions within the promoter region and one transversion in exon region of the pelota gene were detected in the parental lines. However, the relative transcript levels of pelota did not significantly differ among the three tomato lines, regardless of TYLCV infection. This study will facilitate marker-assisted breeding for resistance to TYLCV and lay a foundation for the research of the resistance mechanism of ty-5 in tomato.
Highlights
Tomato yellow leaf curl virus (TYLCV) has significantly impacted the tomato industry around the world, and the use of insecticides and insect nets have not effectively controlled the spread of this pathogen
We have identified a recessive TYLCV resistance gene, hereby designated as ty-5, which is linked to SlNACI
In contrast to the high disease severity index (DSI) results for the control tomato line Money maker that was susceptible to infection, all the other lines were highly resistant to TYLCV using both inoculation methods
Summary
Tomato yellow leaf curl virus (TYLCV) has significantly impacted the tomato industry around the world, and the use of insecticides and insect nets have not effectively controlled the spread of this pathogen. The tomato line AVTO1227 is highly resistant to TYLCV. The primary strategies for controlling TYLCV transmission involve controlling populations of the whitefly vector and choosing tomato cultivars that are resistant to the virus. Planting of cultivars that are more resistant to TYLCV infection has become a more prominent strategy to maintain tomato production[10]. Ty-6 is a novel resistance gene that is located on chromosome 10 and was derived from S. chilense strain LA277919. Of the above resistance genes, Ty-1, Ty-2, and Ty-3 have been primarily introgressed into hybrid tomato cultivars in China and have prevented substantial TYLCV-related losses in the tomato industry. We conducted WGR of two genomic DNA pools representing tomatoes that are resistant and susceptible to TYLCV and were developed from plants in a TYLCV-inoculated F2 population.
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