Abstract

This paper aimed to showing the effects of sewage water and processed water on antioxidant enzymes of broad bean, wheat, and radish plants. The results showed that, sewage and processed water induced significant increasing in catalase activity (CAT) in root (vegetative) and in most cases of the fruiting stage in broad bean and wheat but, in radish both sewage and processed water increased the CAT activity. Sewage and processed water induced slight increase in SOD activity in most cases of bean but the reverse in wheat, where the increasing was in vegetative stage but SOD reduced in fruiting in radish highly increase in vegetative root but in vegetative shoot and fruiting root the increase in SOD activity was lowered, SOD activity in fruiting shoot was reduced. Glutathione reductase was reduced in most cases of broad bean but increased significantly in wheat plant under irrigation with both sewage and processed water. In radish G.R. nearly not affected except in vegetative shoot it increased significantly and reduced but reduced significantly in fruiting shoot with both of sewage and processed water. POD and APX activity was increased markedly in all tested plants in most cases with irrigation by sewage and processed water except in vegetative shoot and fruiting root in radish were reduced. As a result MDA content in both broad bean and wheat plants was reduced in most cases when plants treated with both sewage and processed water but in radish they were around control (increase/ or decrease) in most cases . Also we determined the heavy metals content of the tested plants where, Fe content increased by sewage and processed water irrigation in broad bean and wheat but in radish sewage water reduced Fe content but processed water increased Fe content in fruiting stage. Mn content increased in most organs of broad bean and radish irrigated with sewage water but reduced when they was irrigated with processed water, the reverse was found in wheat. Ni content in broad bean reduced in fruiting stage after irrigation with both sewage and processed water, but increased in wheat nearly the same in radish. Cd content in fruiting stage (especially in seeds) was disappeared when tested plants irrigated by processed water. New Egyptian Journal of Microbiology Vol. 17 (2) 2007: pp. 40-52

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