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Abstract
The application of three different vitrification-based freezing strategies for the cryostorage of white poplar (Populus alba L.) and hybrid aspen (P. tremula L. × P. tremuloides Michx.) have been assessed. The PVS2 vitrification protocol was successfully applied to two white poplar in vitro clones stored for more than 6 months in slow-growth conditions (4 °C, in darkness) and showing clear signs of explant etiolation and decay. After 60 min of PVS2 treatment, P. alba L. (cv. Villafranca) explants isolated from axillary buds demonstrated significantly better potential for post-freeze regrowth (64%) compared to those obtained from apical buds (17%). Similarly, a high level of survival (78%) of the frozen hybrid aspen shoot tips was recorded following the application of the same technique. Using the encapsulation-vitrification procedure, no toxic effects of the PVS2 treatment were noticed after 120 min exposure, however none of the cryopreserved (poplar and aspen) explants survived after 3 weeks. In contrast, the droplet-vitrification technique appeared to be very efficient in the cryopreservation of white poplar shoot tips, which increases the opportunities for wider application of this method in other woody species.;
Highlights
IntroductionThe poplar (Populus spp.) is rapidly being adopted as a model for studying different aspects of forest tree genetics for a number of reasons: its relatively small genome size, considerable genetic variation (both natural and resulting from breeding activity), fast juvenile growth, ease of in vitro clonal propagation and efficient transformation (Taylor 2002)
The poplar (Populus spp.) is rapidly being adopted as a model for studying different aspects of forest tree genetics for a number of reasons: its relatively small genome size, considerable genetic variation, fast juvenile growth, ease of in vitro clonal propagation and efficient transformation (Taylor 2002)
No post-freeze survival was achieved after the application of encapsulation-vitrification technique to both the white poplar ‘CSM’ and ‘MLF’ clones
Summary
The poplar (Populus spp.) is rapidly being adopted as a model for studying different aspects of forest tree genetics for a number of reasons: its relatively small genome size, considerable genetic variation (both natural and resulting from breeding activity), fast juvenile growth, ease of in vitro clonal propagation and efficient transformation (Taylor 2002). Poplars are common throughout the northern hemisphere and well-adapted to a broad range of environments and climatic conditions. L.) is native to Central and Southern Europe and found across Western Siberia and Central Asia in the riparian zones, as well as in natural forests. The species is appreciated for its ornamental value and broadly cultivated in urban areas. Among the white poplar cultivars in commercial use for years, the cv. Villafranca is that which has been intensively exploited in the production of timber and as a potential candidate for establishing ‘energy biomass’
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