Application of UV‐curable diazoresin. I. Immobilization of glucose oxidase into PVA in the presence of UV‐sensitive diazoresin and sensitizers

Abstract

AbstractA study of the immobilization of glucose oxidase into poly(vinyl alcohol) membrane in the presence of UV light sensitive diazcresin and benzoic acid and/or sodium benzoate sensitizers was carried out. An effective enzyme electrode by using the immobilized glucose oxidase membrane was developed. The effect of the concentration of diazoresin and sensitizers in poly(vinyl alcohol), UV nght irradiation on the degree of insolubility as well as the activity yield of the membrane were examined for the immobilized glucose oxidase using glucose as a substrate. An unstable phenomenon was found in the initial usage of the immobilized glucose oxidase membrane prepared from poly(vinyl alcohol)‐sensitizer system. Activity yields of 39.98 and 35.30 for poly(vinyl alcohol)‐diazoresin and poly(vinyl alcohol)‐sensitizer system were obtained, respectively.