Abstract
Objectives:To study the ability of aptamer-modified nano-gold rods and liquid carbon-targeted PLGA nanoparticles to target in vitro using compressed sensing reconstruction algorithm, and observe the phenomenon of mediating ultrasound / photoacoustic imaging.Methods:PLGA nanoparticles were prepared by a double emulsification method, and the MUC1 aptamer was connected to the PLGA nanoparticles by the carbodiimide method to obtain an “aptamer-PLGA nanoparticle” targeted phase change contrast agent. Fluorescence microscopy was used to detect the in vitro targeting of breast cancer MCF-7 cells specifically identified by it, and three control groups were set up: the ordinary nanoparticle group, the aptamer interference group, and the HELA cell group. A photoacoustic instrument was used to observe the phenomenon of enhanced ultrasound / photoacoustic signal mediated in vitro.Results:Many targeted nanoparticles were clustered around MCF-7 cells and bound firmly, but no specific binding was observed in the non-targeted nanoparticles group, the aptamer interference group and the HELA cell group. After the targeted nanoparticle was excited by the photoacoustic instrument, the ultrasonic signal and the photoacoustic signal were significantly enhanced compared with before the excitation.Conclusion:The successfully prepared targeting nanoparticles have good targeting and specificity for breast cancer MCF-7 cells, and it has obvious effects on ultrasound / photoacoustic imaging, and has the potential to become a dual-mode ultrasound / photoacoustic targeted contrast agent. The various characteristics provide experimental basis for subsequent in vivo targeting experiments and are expected to become good target diagnostic molecular probes.
Highlights
Breast cancer is a common oncological disease among women all over the world, and 42% of female cancer cases worldwide occur in developing countries.[1,2] Breast cancer and tumors are seriously affecting people’s lives
Experimental methods: Preparation of aptamerPLGA nanoparticle-targeted contrast agent: (1) Preparation of PLGA nanoparticles coated with nano-gold rods and perfluoro hexane (PFH): a mixture containing 200μl of PFH and 1 ml of nano-gold rods was shaken with a vibrator 1 A purple emulsion was obtained after few minutes
Inverted fluorescence microscopy showed that both the MCF-7 cells labeled with Dia and HELA cells were green, and the aptamer-PLGA” targeted nanoparticles (AP-GNP) and GNP labeled with Dli were red
Summary
Breast cancer is a common oncological disease among women all over the world, and 42% of female cancer cases worldwide occur in developing countries.[1,2] Breast cancer and tumors are seriously affecting people’s lives. Image diagnosis plays a vital role in cancer diagnosis.[3,4] With the rapid development of molecular imaging technology, molecular-level imaging has laid a solid foundation for early diagnosis and treatment of various diseases.[5,6] Targeted multi-modal. Pak J Med Sci September 2021 (Speical Issue Online) Vol 37 No 6 www.pjms.org.pk 1610 contrast agents have attracted much attention because they can be used in multiple imaging modes to obtain relatively complete biological information and highly specific recognition of targets.[7,8] Photoacoustic imaging is a new type of non-destructive medical imaging mode based on photoacoustic effects.[9,10] It combines the advantages of pure optical imaging with high contrast and ultrasonic imaging with high penetration depth. It has been under discussion in recent years.[11,12,13]
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