Abstract
We have developed one approach to fingerprint and estimate the active compounds in the leaves of Vitex trifolia Linn possessing anti-tracheospasmolytic assay using two-dimensional TLC pattern comparison. Based on the two-dimensional TLC pattern and the activity of the centrifugal partition chromatography fractions, we concluded that the semi polar compounds were responsible for anti-tracheospasmolytic activity. The best non-polar/semi polar mobile phases for the two-dimensional TLC using silica gel as the stationary phase were chloroform/methanol (9/1) as the first mobile phase and ethyl acetate/chloroform/methanol (28/28/44) as the second mobile phase.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
