Application of two-dimensional CSI for absolute quantification of phosphorus metabolites in the human liver

Abstract

There have recently been a number of studies dealing with the absolute quantification of concentrations of MR-visible phosphorus compounds in different tissues. The use of absolute values rather than intensity ratios may furnish additional information about metabolic changes associated with different diseases. The purpose of this study was to develop a general procedure for measuring molar metabolite concentrations and to apply it with respect to the evaluation of human liver 31P-MRS data measured using a standard slice-selective two-dimensional CSI sequence and commercial 1H/31P surface coil. The experimental determination of all surface coil-related factors influencing signal intensity was undertaken using a gradient echo imaging technique that can be adapted to commercial systems. The resulting values for healthy volunteers (N = 9) showed concentrations of PME = 2.8 +/- 1.3 mM, PDE = 9.9 +/- 2.7 mM, P(i) = 1.7+/-0.7 mM, and ATP = 3.6 +/- 0.9 mM in the human liver. The data are quite consistent with published findings.