Abstract
A high-performance liquid chromatography (HPLC) method has been developed for assays of five barbiturates in human urine using a new thermoresponsive polymer separation column, which is composed of poly(N-isopropylacrylamide). By elevating the column temperature from 10°C to 50°C, the barbiturates metharbital, primidone, phenobarbital, mephobarbital, and pentobarbital became well separated by this method. The five barbiturates showed good linearity in the range of 0.2–10 μg/ml. Good accuracy, precision, and recoveries for these drugs were obtained at 1 and 5 μg/ml urine. The method with this new column type seems to have high potential for extensive use in forensic toxicology for analysis of many drugs and poisons by HPLC and HPLC-mass spectrometry.
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