Application of the VSP near-infrared fluorescence probe in hyperlipidemia viscosity monitoring

Abstract

This study introduces a novel near-infrared fluorescent probe named VSP, designed to effectively and sensitively monitor viscosity changes both internally and externally. Through an enhanced conjugation structure of the fluorescent molecule and the incorporation of hydrophilic groups, the VSP probe showcases remarkable sensitivity, selectivity, and stability in its ability to detect viscosity alterations. Notably, the linear correlation between its fluorescence intensity and glycerol content presents the possibility of quantitative viscosity detection, holding significant promise for applications in biomedical research and clinical diagnostics. Moreover, its biocompatibility supports its use for monitoring viscosity within cellular environments. An exceptional attribute is its ability to accurately distinguish between hyperlipidemic model mice and normal mice in animal imaging as well as serum samples, underscoring its potential for early-stage diagnosis of elevated lipid levels and risk assessment for cardiovascular diseases. VSP's potential was demonstrated in cell imaging and differentiation between high-fat and normal mice based on blood serum viscosity. This promising tool holds significant potential for biomedical research and clinical applications in monitoring and analyzing changes in biological systems.