Abstract
Application of the RAPD and miRNA markers in the genotyping of Silybum marianum (L.) Gaertn.
Highlights
IntroductionGaertn., or milk thistle, is a medicinal plant of unique pharmaceutical properties for treating liver diseases, used as a cytoprotectant, anticarcinogen and for the treatment of diabetic nephropathy (RafieianKopaie and Nasri, 2012; Mohammadi et al, 2011; Rainode, 2005)
The total genomic DNA was extracted from the leaves of 10-day old seedlings using three different commercial plant DNA isolation kits and two complex methods, the cetyltrimethylammonium bromide (CTAB) method described by Rogers and Bendich (1994) and the modified CTAB extraction applied for medicinal plants by Padmalatha and Prasad (2006)
The molecular markers used in this research were applied in order to obtain the fingerprinting of individual genotypes of Silybum marianum L
Summary
Gaertn., or milk thistle, is a medicinal plant of unique pharmaceutical properties for treating liver diseases, used as a cytoprotectant, anticarcinogen and for the treatment of diabetic nephropathy (RafieianKopaie and Nasri, 2012; Mohammadi et al, 2011; Rainode, 2005). The active complex of milk thistle is a lipophilic extract from plant seeds and it is composed of three isomer flavonolignans (silybinin, silydianin and silychristin) collectively known as silymarin. Silymarin is found in the entire plant but it is concentrated in the fruit and seeds. Silybum marianum is the most cultivated medicinal plant in Slovakia. The long-term interest in the cultivation of this medicinal plant is significant in terms of its silymarin complex for the production of pharmaceuticals (Habán and Vaverková, 2014; Habán et al, 2015)
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