Application of the method of electro-acoustical analysis for the detection of bacteriophages in a liquid phase

Abstract

The possibility of the application of electro-acoustic analysis for the detection of bacteriophages was demonstrated for the first time based on the example of the interaction of the FA1-Sp59b bacteriophage with bacterial cells of the strain Azospirillum lipoferum Sp59b. Piezoelectric cross-field resonators with a 1-mL chamber for analyzed liquid were used as the biological sensor. It was revealed that the dependences of the real and imaginary parts of the electrical impedance of the resonator loaded with a suspension of viruses and microbial cells on the frequency was significantly different from those dependences of the resonator that contained a control cell suspension without the virus. It was shown that detection of the FA1-Sp59b bacteriophage using microbial cells was possible with both extraneous viral particles and extraneous microbial cells. The proposed method allows one to accurately determine the type of identified virus after a 5-minute interaction with indicating bacterial culture. As well, the minimum concentration of viruses is five virus particles per cell. These results as a whole demonstrate the possibility of detecting specific interactions of bacteriophages with microbial cells and provide a basis for the development of a biological sensor for the quantitative detection of viruses directly in the liquid phase.