Abstract
The study focuses on the analysis of a sensitive, selective, and simple postcolumn detection method for thioguanine determination, based on the sensitizing induction of thioguanine on iodine-azide reaction and the combination technique of HPLC. The analysis was accomplished in the optimum conditions for iodine-azide detection system and HPLC separation. The values for the linear range, the LOD, and DOQ amounted to 0.8-1.7, 0.4, and 0.5 nmol/mL urine, respectively.
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