Abstract
Application of a direct immunoperoxidase technique for the detection of Derzsy's disease virus antigen in cell culture and goslings is described. Anti-virus globulins were labelled with horseradish peroxidase by use of sodium periodate as a coupling agent. Virus antigen was detected in the nuclei of goose embryo fibroblasts from 48 to 72 hours post-inoculation and in the nuclei of hepatic cells between 5 to 8 days after infection. Microstructural detail was much better in cell culture than in the liver.
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