Abstract
Simple SummaryThe study was conducted with the aim of visualizing sex chromosomes in domestic cat sperm. Our research was carried out using fluorescence in situ hybridization (FISH) technique and the microscopic analysis showed the presence of X and Y chromosomes in domestic cat spermatozoa. The procedure used for sperm decondensation and FISH is adequate to visualize chromosomes in domestic cat sperm.Fluorescence in situ hybridization is a molecular cytogenetics technique that enables the visualization of chromosomes in cells via fluorescently labeled molecular probes specific to selected chromosomes. Despite difficulties in carrying out the FISH technique on sperm, related to the need for proper nuclear chromatin decondensation, this technique has already been used to visualize chromosomes in human, mouse, cattle, swine, horse, and dog spermatozoa. Until now, FISH has not been performed on domestic cat sperm; therefore, the aim of this study was to visualize sex chromosomes in domestic cat sperm. The results showed the presence of X and Y chromosomes in feline spermatozoa. The procedure used for sperm decondensation and fluorescence in situ hybridization was adequate to visualize chromosomes in domestic cat spermatozoa and, in the future, it may be used to determine the degree of chromosomal abnormalities in these gametes.
Highlights
Disturbed segregation of chromosomes during gametogenesis can lead to the formation of gametes with an abnormal number of chromosomes
Fluorescence in situ hybridization (FISH) is a molecular cytogenetics technique that uses fluorescently labeled molecular probes specific to selected chromosomes to enable their visualization in cells
Study,the thesex sexchromosomes chromosomes domestic spermatozoa were visualized for first time following the application of the whole-chromosome the first time following the application of the FISH technique with X and Y whole-chropainting (WCPP), labeled with fluorochromes
Summary
Disturbed segregation of chromosomes during gametogenesis can lead to the formation of gametes with an abnormal number of chromosomes. Fertilization with such a gamete may result in the formation of embryos with mutations leading to embryo death or spontaneous abortion [1,2,3]. There are studies in the literature on the use of the FISH technique in humans [10,11], mice [12,13], rats [14], cattle [5,9,15,16], swine [17,18,19,20], horses [4,5,21], and dogs [22,23], FISH has not yet been performed on domestic cat sperm. The aim of this study was to visualize the sex chromosomes in domestic cat sperm
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