Abstract
A cloning method utilizing the enzyme histochemical procedure was applied to isolate variant HeLa subclones which produce different alkaline phosphatase isozymes. Phosphate was used to distinguish between Kasahara isozyme and Regan isozyme. The use of filter paper made it possible to determine the localization of the Kasahara isozyme-positive colonies. By this method, two variant clones, HeLa S3-10 KP and HeLa S3-10 KN, were isolated. Kasahara isozyme was induced in the former by increased cell density, but not in the latter.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
