Abstract
The Davidon-Fletcher-Powell (DFP) iterative algorithm was used for the simultaneous quantitation of the components of complex mixtures from their UV-vis spectra. First, the effect of noise, overlap between standard spectra and the starting point for resolving numerically generated spectra were investigated. Then, spectra for the pure solutions of the mixture components were used to apply the method to the resolution of ternary and quaternary mixtures of active principles and pharmaceutical preparations. Samples were also quantified by using a set of standard spectra which included not only those of the pure components, but also some for other substances. The proposed method effectively quantifies the actual mixture components.
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