Abstract
High-risk papillomavirus (HR-HPV) testing combined with cytology improves the detection of cervical lesions and increases length of screening intervals. For a population-based HR-HPV survey, testing automation is in great need. The Cobas 4800 HPV Test System is a fully automated assay that can simultaneously detect HPV16, HPV18, and other 12 pooled HR-HPV genotypes. This system has been employed for HR-HPV screening in a number of countries; however, such application in a large population in China has not been documented. In this study, we employed the Cobas 4800 HPV Test System to detect HR-HPV in cervical cytology specimens collected from a total of 5650 asymptomatic women from a region of South China. We reported the following: (1) the prevalence of the 14 genotypes of HR-HPV was 12.96%; (2) for those with HR-HPV infection, 2.25% were positive for HPV16, 0.50% for HPV18, 9.15% for pooled 12 HPV types, and 1.06% for multiple HPV infection; and (3) there was no significant difference in the HR-HPV prevalence among different age groups. HPV16 and HPV18 have been shown to be the predominant HPV types found in cervical cancer patients in some regions in China, indicating that a fully automated assay like the Cobas 4800 HPV Test System is especially valuable for population-based HR-HPV screening in these regions as this assay can concurrently detect HPV16 and HPV18.
Highlights
Human papillomavirus (HPV) is a double-stranded DNA virus of the Papillomaviridae family [1]
Multiple HPV infections include HPV16 mixed with HPV18, HPV16 mixed with 12 pooled types, HPV18 mixed with 12 pooled types, or HPV16 mixed with HPV18 and 12 pooled types
732 (12.96%) were detected as high-risk HPV (HR-HPV)-positive, including 127 (2.25%) with HPV16 infection, 28 (0.50%) with HPV18, 517 (9.15%) with pooled 12 HPV types, and 60 (1.06%) with multiple HPV infection (HPV16 mixed with HPV18, HPV16 mixed with 12 pooled types, HPV18 mixed with 12 pooled types, or HPV16 mixed with HPV18 and 12 pooled types) (Table 1)
Summary
Human papillomavirus (HPV) is a double-stranded DNA virus of the Papillomaviridae family [1]. There are more than 200 genotypes of HPV that have been identified, among which 15 (16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 68, 73, and 82) are highly associated with cervical cancer [2, 3]. To reduce the burden of cervical cancer, early identification of cervical lesions that likely progress to invasive cancer is of paramount importance. Cervical cytology plays an important role in the prevention of cervical cancer [6]. The addition of HPV testing to cytology results in an increased detection of high-grade cervical lesions, and it is recommended that screening of high-risk HPV (HR-HPV) be incorporated into cytology to improve disease detection and increase length of screening intervals [7, 8]
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