Abstract
In an attempt to localize cellulase activity at the electron microscope level, in aging quiescent root meristems of Allium cepa L, the end product (reducing sugar) of the substrate was allowed to reduce cupric salts into cuprous oxide, which resulted in localized dense deposits in the cell walls. In the absence of any data regarding the elemental composition of these deposits, it was necessary to use the facilities provided by the Analytical Electron Microscope, EMMA-4. This instrument, by the combination of conventional transmission images and X-ray microanalytical facilities, allows unambiguous identification of elements in cell components. The analytical devise of EMMA-4 consists of two fully focussing crystal spectrometers capable of high resolution wavelength dispersive analysis and either a gas flow non-dispersive detector or a solid state Si (Li) non-dispersive detector for energy dispersive analysis.
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Schedule a callMore From: Proceedings, annual meeting, Electron Microscopy Society of America
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