Application of the adenosine triphosphate sensitivity assay in infantile vascular anomalies

Li Li,Bin Zhang,Li Wei,Zi-Gang Xu,Lin Ma,Xiao-Feng Han,Bin Yang

doi:10.1186/s12887-020-1974-9

Li Li, Bin Zhang + Show 5 more

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https://doi.org/10.1186/s12887-020-1974-9

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Abstract

BackgroundThe term vascular anomalies include various vascular tumors and vascular malformations, among them infantile hemangiomas and capillary malformations are the most well-known associated diseases in early ages. Multiple drugs have been introduced for intervention, but susceptibility test in vitro were scarcely reported.ObjectiveTo evaluate the inhibition effect of different drugs by adenosine triphosphate sensitivity assay in vitro before the treatment of infantile hemangiomas and capillary malformations.MethodsSpecimens were selected from 5 cases of infantile hemangiomas and 11 cases of capillary malformations. Propranolol, rapamycin, sildenafil and itraconazole were tested for their growth inhibition effect by using the adenosine triphosphate sensitivity assay.ResultsPropranolol demonstrated inhibitory effects on infantile hemangiomas cells. Rapamycin and itraconazole both showed inhibitory effects on infantile hemangiomas cells and capillary malformations cells. Sildenafil has no growth inhibitory effect on infantile hemangiomas cells or capillary malformations cells.ConclusionAdenosine triphosphate sensitivity assay is a sensitive and useful testing method before the management of vascular anomalies, and individualized medication suggestions for the choice of therapeutic drugs were offered based on the testing result and together with a comprehensive evaluation of each infant.

Highlights

The term vascular anomalies include various vascular tumors and vascular malformations, among them infantile hemangiomas and capillary malformations are the most well-known associated diseases in early ages
In recent decades, the International Society for the Study of Vascular Anomalies (ISSVA) developed a classification system and these anomalies are divided into vascular tumors and vascular malformations based on their biology and genetics [2]
Vascular malformations are frequently occurred in infants, of which capillary malformations are the most common entities [5]

Summary

Introduction

The term vascular anomalies include various vascular tumors and vascular malformations, among them infantile hemangiomas and capillary malformations are the most well-known associated diseases in early ages. Infantile hemangiomas are the most common vascular anomalies in infants [3], and it is the most common benign neoplasm in infants. Vascular malformations are frequently occurred in infants, of which capillary malformations (naevus flammeus, port wine stains) are the most common entities [5]. There are multiple available treatment prescriptions or approaches for vascular anomalies, including drug therapy (such as glucocorticoids, antineoplastic chemotherapeutic agents, interferon, etc.), physical therapy (such as laser [6], freezing, radiotherapy, radionuclide, radioisotope, and shallow X-ray radiation), surgical intervention, electrochemical therapy, and so on [7].

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