Abstract
Quantum dots (QDs) have received considerable attention due to their advantages and are widely used in biological studies, especially for multiplexed staining assays and immunological assays. Here we report an easy method for quantum dot synthesis and encapsulation, and use for efficient bioconjugation with secondary antibody. For the application of QDs-antibody conjugates, we approached a fast dot blotting immunological assay, and then we used a breast cancer cell line SKBR3 and anti-EpCAM antibody as the primary antibody for the cell imaging. The results shows that this conjugates can be successfully used in immunological assays such as dot blotting and cell imaging, and with the help of certain blocking buffers, specific and sensitive signals can be obtained.
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