Abstract
SnO2 nanocrystal (NC) was applied as a novel ECL signal reporter for sandwich-type electrochemiluminescence (ECL) detection of carcinoembryonic antigen with nanoporous PtRu alloy enhancement. For immobilizing more SnO2 NCs, nanoporous PtRu alloy were synthesized by a selective dissolution of Al from PtRuAl alloy in NaOH, which has large area-to-volume ratio. And their interconnected skeletons are very favorable for electron transport, which can benefit for the ECL behavior. So the composite of SnO2 NCs functionalized PtRu was used to label signal antibody to obtain enhanced ECL performance. And nanoporous silver was employed as a primary antibody carrier for our designed immunosensor based on a disposable microdevice. The resulting versatile immunosensor possesses high sensitivity, satisfactory reproducibility and stability, and good specificity.
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