Application of Silver Staining for Proteins in Rapid Staining of Undecalcified Tooth Sections

Abstract

A convenient silver staining method generally used in marking proteins in polyacrylamide gel was applied to stain undecalcified tooth sections for clear observation of fine structures in tooth tissues. Ground sections of teeth were directly soaked and stained with a solution prepared according to the staining protocol. Although modification of the staining procedure might be necessary to establish favorable staining condition in tooth sections, observation of fine structures in tooth sections became easy with the staining technique.