Application of Serological Tools and Spatial Analysis to Investigate Malaria Transmission Dynamics in Highland Areas of Southwest Uganda.

Caroline A Lynch,Chris Drakeley,Jackie Cook,Patrick Corran,Jonathan Cox,Sarah Nanyunja,Cally Roper,Jane Bruce,Richard Pearce,John B Rwakimari,Tarekegn A Abeku,Amit Bhasin

doi:10.4269/ajtmh.15-0653

Abstract

Serological markers, combined with spatial analysis, offer a comparatively more sensitive means by which to measure and detect foci of malaria transmission in highland areas than traditional malariometric indicators. Plasmodium falciparum parasite prevalence, seroprevalence, and seroconversion rate to P. falciparum merozoite surface protein-119 (MSP-119) were measured in a cross-sectional survey to determine differences in transmission between altitudinal strata. Clusters of P. falciparum parasite prevalence and high antibody responses to MSP-119 were detected and compared. Results show that P. falciparum prevalence and seroprevalence generally decreased with increasing altitude. However, transmission was heterogeneous with hotspots of prevalence and/or seroprevalence detected in both highland and highland fringe altitudes, including a serological hotspot at 2,200 m. Results demonstrate that seroprevalence can be used as an additional tool to identify hotspots of malaria transmission that might be difficult to detect using traditional cross-sectional parasite surveys or through vector studies. Our study findings identify ways in which malaria prevention and control can be more effectively targeted in highland or low transmission areas via serological measures. These tools will become increasingly important for countries with an elimination agenda and/or where malaria transmission is becoming patchy and focal, but receptivity to malaria transmission remains high.

Highlights

In the east African highland areas, malaria transmission intensity generally decreases with altitude, often becoming heterogeneous as altitude increases, to a point where malaria is no longer transmitted.[1,2,3,4,5,6] The main drivers behind these changes are thought to be a decrease in temperature and humidity that results in decreased mosquito vector density as altitude increases
Antibodies to P. falciparum merozoite surface protein-119 (MSP-119) were detected in the blood eluted from the filter paper blood spots by an indirect enzyme-linked immunosorbent assay (ELISA) using recombinant P. falciparum MSP-119.26 Sera were tested at a single dilution (1:1,000) and a positive control curve of hyperimmune sera on each plate was used to standardize results between ELISA plates
A total of 2,125 individuals were sampled, all of whom were tested for parasite infections using Paracheck Pf rapid diagnostic test (RDT) and 1,919 were tested for MSP-119 using ELISA

Summary

Introduction

In the east African highland areas, malaria transmission intensity generally decreases with altitude, often becoming heterogeneous as altitude increases, to a point where malaria is no longer transmitted.[1,2,3,4,5,6] The main drivers behind these changes are thought to be a decrease in temperature and humidity that results in decreased mosquito vector density as altitude increases. Clusters or hotspots of relatively high malaria transmission have been detected in highland areas, often associated with proximity to vector breeding sites such as forests, natural swamps, highland floodplains, or farmlands and pastures.[7,8,9,10,11,12,13,14] Over a highland landscape, the heterogeneity in distribution of malaria can reflect microclimates suitable for vector breeding, coupled with differences in household structures or genetic factors.[15,16,17,18]. The World Health Organization has previously defined foci of malaria as localities with continuous or intermittent epidemiological factors necessary for transmission.[19] Bousema and others defined a hotspot as a geographical part of a focus where malaria transmission exceeds the average level in surrounding areas.[15]

Methods

Results

Conclusion